Daystar University was first housed at the Nairobi Campus. This campus is located  approximately two kilometres from the City Centre, at the corner of Ngong Road and Valley Roads.

It is a few minutes walk from Hurlingham shopping centre, the Kenyatta National Hospital and Nairobi Hospital. It also neigbours Nairobi Baptist Church and is easily accessible by public and private means.

The campus hosts the diploma programme, day and evening undergraduate programs and the post-graduate programs in Communication, Theology and Business. At this campus, the university also offers short courses in various areas including communication, community development and business.